Raw Data Statistics

  Quality of the raw data was assessed to check fidelity of the rawdata. Q20 or Q30 represent portion of the reads having quality equal or higher than 20 or 30 phread score, respectively. Phred score of 20 means the base has 99% accuracy. The reads of which Q20 bases occupy 90% are generally accepted as good quality reads for analysis and it is sure that more higher Q20 or/and Q30 enable to get more accurate analysis results. GC contents represent portion of nucleotide GC and AT. In most cases, GC contents of metagenome samples shows half and half because metagenome samples contain various species. If GC or AT contents are much higher than others, it means that GC or AT rich species occupy majority of the metagenome samples.

•     · Total Yield (bp) : Total number of bases sequenced.
•     · Total reads : Total Number of Reads.
•     · GC (%) : GC content.
•     · Q20 (%) : Ratio of bases that have phred quality score of over 20.
•     · Q30 (%) : Ratio of bases that have phred quality score of over 30.
•     · Base Quality Plot : This plot shows the average, interquartile range and median quality of each base.

  The raw data were pre-processed using Trimmomatic which can be used to trim adapter sequences and end-base low quality sequences. Quality of the filtered data was assessed to check fidelity of the filtered data. Q20 or Q30 represent portion of the reads having quality equal or higher than 20 or 30 phread score. Phred score of 20 means the base has 99% accuracy. The reads of which Q20 bases occupy 90% are generally accepted as good quality reads for analysis and it is sure that more higher Q20 or/and Q30 enable to get more accurate analysis results. GC contents represent portion of nucleotide GC and AT. In most cases, GC contents of metagenome samples shows half and half because metagenome samples contain various species. If GC or AT contents are much higher than others, it means that GC or AT rich species occupy majority of the metagenome samples.

•     · Total Yield (bp) : Total number of bases sequenced.
•     · Total reads : Total Number of Reads.
•     · GC (%) : GC content.
•     · Q20 (%) : Ratio of bases that have phred quality score of over 20.
•     · Q30 (%) : Ratio of bases that have phred quality score of over 30.
•     · Base Quality Plot : This plot shows the average quality of each base.

  Q20 or Q30 represent portion of the reads having quality equal or higher than 20 or 30 phread score. High Q20 or/and Q30 mean that the raw data have high quality and good enough for further analysis.

  GC contents represent portion of nucleotide GC and AT. In most cases, GC contents of metagenome samples shows half and half because metagenome samples contain various species. If GC or AT contents are much higher than others, it means that GC or AT rich species occupy majority of the metagenome samples.