Si tu primer está en esta lista no necesitas enviarlo: lo aplicamos sin coste adicional a tu reacción Sanger. Cubrimos los vectores y barcodes más comunes: M13, T7, SP6, T3, BGH, CMV, EGFP, AOX1, ITS1–5, 16S (27F/1492R) y COI (LCO/HCO), entre otros.
| # | Nombre del primer | Secuencia (5'→3') | Longitud (bp) |
|---|---|---|---|
| 1 | Bluescript_SK | CGCTCTAGAACTAGTGGATC | 20 |
| 2 | Bluescript_KS | TCGAGGTCGACGGTATC | 17 |
| 3 | KAN2-FP | ACCTACAACAAAGCTCTCATCAACC | 25 |
| 4 | KAN2-RP | GCAATGTAACATCAGAGATTTTGAG | 25 |
| 5 | pBacPAC-RP | GTCTGTAAATCAACAACGC | 19 |
| 6 | pBAD-FP | ATGCCATAGCATTTTATCC | 20 |
| 7 | pDONOR-FP | TAACGCTAGCATGGATCTC | 19 |
| 8 | pEGFP_N | CCGTCCAGCTCGACCAG | 17 |
| 9 | pEGFP-FP | TTTAGTGAACCGTCAGATC | 19 |
| 10 | pEGFP-RP | AACAGCTCCTCGCCTTG | 18 |
| 11 | pESP-RP | TCCAAAAGAAGTCGAGTGG | 19 |
| 12 | pET-24a | GGGTTATGCTAGTTATTGCTCAG | 23 |
| 13 | pET-RP | CTAGTTATTGCTCAGCGG | 18 |
| 14 | pMalE | TACGACTGTCGATGAAGC | 18 |
| 15 | pREP-fwd | GCTCGATACAATAAACGCC | 19 |
| 16 | 35S-A | AAGGGTCTTGCGAAGGATAG | 20 |
| 17 | 35S-B | AGTGGAAAAGGAAGGTGGCT | 20 |
| 18 | AD_Reverse | AGATGGTGCACGATGCACAG | 20 |
| 19 | a-Factor | TACTATTGCCAGCATTGCTGC | 21 |
| 20 | CYC1_Reverse | GCGTGAATGTAAGCGTGAC | 19 |
| 21 | DsRed1-C | AGCTGGACATCACCTCCCACAACG | 24 |
| 22 | DsRed1-N | GTACTGGAACTGGGGGGACAG | 21 |
| 23 | EBV-RP | GTGGTTTGTCCAAACTCATC | 20 |
| 24 | EGFP-C | CATGGTCCTGCTGGAGTTCGTG | 22 |
| 25 | EGFP-N | CGTCGCCGTCCAGCTCGACCAG | 22 |
| 26 | U-19mer_Primer | GTTTTCCCAGTCACGACGT | 19 |
| 27 | T7 EEV | ATGTCGTAATAACCCCGCCCCG | 22 |
| 28 | pFastBac_Forward | GGATTATTCATACCGTCCCA | 20 |
| 29 | pFastBac_Reverse | CAAATGTGGTATGGCTGATT | 20 |
| 30 | AOX1_Forward | GACTGGTTCCAATTGACAAGC | 21 |
| 31 | AOX1_Reverse | GCAAATGGCATTCTGACATCC | 21 |
| 32 | STag_18mer_Primer | GAACGCCAGCACATGGAC | 18 |
| 33 | MT_Forward | CATCTCAGTGCAACTAAA | 18 |
| 34 | QE_Promoter | CCGAAAAGTGCCACCTG | 17 |
| 35 | pRH_Forward | CTGTCTCTATACTCCCCTATAG | 22 |
| 36 | pRH_Reverse | CAAAATTCAATAGTTACTATCGC | 23 |
| 37 | pTrcHis_Forward | GAGGTATATATTAATGTATCG | 21 |
| 38 | pJET1.2F | CGACTCACTATAGGGAGAGCGGC | 23 |
| 39 | pJET1.2R | AAGAACATCGATTTTCCATGGCAG | 24 |
| 40 | SV40-pArev | CCTCTACAAATGTGGTATGG | 20 |
| 41 | SV40-Promoter | GCCCCTAACTCCGCCCATCC | 20 |
| 42 | SP6 | ATTTAGGTGACACTATAG | 18 |
| 43 | T3 | ATTAACCCTCACTAAAG | 17 |
| 44 | T7 | AATACGACTCACTATAG | 17 |
| 45 | T7terminator | GCTAGTTATTGCTCAGCGG | 19 |
| 46 | T7promoter | TAATACGACTCACTATAGGG | 20 |
| 47 | M13-FP | TGTAAAACGACGGCCAGT | 18 |
| 48 | M13F-pUC(-40) | GTTTTCCCAGTCACGAC | 17 |
| 49 | M13R-pUC(-40) | CAGGAAACAGCTATGAC | 17 |
| 50 | M13F | GTAAAACGACGGCCAGT | 17 |
| 51 | M13R | GCGGATAACAATTTCACACAGG | 22 |
| 52 | pGEX5 | GGCAAGCCACGTTTGGTG | 18 |
| 53 | pGEX3 | GGAGCTGCATGTGTCAGAGG | 20 |
| 54 | BGH-R | TAGAAGGCACAGTCGAGG | 18 |
| 55 | CMV-F | CGCAAATGGGCGGTAGGCGTG | 21 |
| 56 | RVprimer3 | CTAGCAAAATAGGCTGTCCC | 20 |
| 57 | RVprimer4 | GACGATAGTCATGCCCCGCG | 20 |
| 58 | GLprimer1 | TGTATCTTATGGTACTGTAACTG | 23 |
| 59 | GLprimer2 | CTTTATGTTTTTGGCGTCTTCCA | 23 |
| 60 | GAL1_Forward | AATATACCTCTATACTTTAACGTC | 24 |
| 61 | Gal4AD | TACCACTACAATGGATG | 17 |
| 62 | pQE-F | CCCGAAAAGTGCCACCTG | 18 |
| 63 | pQE-R | GTTCTGAGGTCATTACTGG | 19 |
| 64 | pBAD-F | ATGCCATAGCATTTTTATCCA | 21 |
| 65 | pBAD-R | GATTTAATCTGTATCAGG | 18 |
| 66 | EGFP-CF | AGCACCCAGTCCGCCCTGAGC | 21 |
| 67 | EGFP-CR | CGTCCATGCCGAGAGTG | 17 |
| 68 | EGFP-NR | CGTCGCCGTCCAGCTC | 16 |
| 69 | DuetDown1 | GATTATGCGGCCGTGTACAA | 20 |
| 70 | DuetUP2 | TTGTACACGGCCGCATAATC | 20 |
| 71 | pET-upstream | ATGCGTCCGGCGTAGAGG | 18 |
| 72 | LCO1490 | GGTCAACAAATCATAAAGATATTGG | 25 |
| 73 | HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | 26 |
| 74 | ITS1 | TCCGTAGGTGAACCTGCGG | 19 |
| 75 | ITS2 | GCTGCGTTCTTCATCGATGC | 20 |
| 76 | ITS3 | GCATCGATGAAGAACGCAGC | 20 |
| 77 | ITS4 | TCCTCCGCTTATTGATATGC | 20 |
| 78 | ITS5 | GGAAGTAAAAGTCGTAACAAGG | 22 |
| 79 | 27F | AGAGTTTGATCMTGGCTCAG | 20 |
| 80 | 1492R | TACGGYTACCTTGTTACGACTT | 22 |
| 81 | 518F | CCAGCAGCCGCGGTAATACG | 20 |
| 82 | 800R | TACCAGGGTATCTAATCC | 18 |
| 83 | 785F | GGATTAGATACCCTGGTA | 18 |
| 84 | 907R | CCGTCAATTCMTTTRAGTTT | 20 |
| 85 | 1100R | GGGTTGCGCTCGTTG | 15 |
| 86 | 337F | GACTCCTACGGGAGGCWGCAG | 21 |
| 87 | NS1 | GTAGTCATATGCTTGTCTC | 19 |
| 88 | NS8 | TCCGCAGGTTCACCTACGGA | 20 |
| 89 | NS24 | AAACCTTGTTACGACTTTTA | 20 |
| 90 | LR0R | ACCCGCTGAACTTAAGC | 17 |
| 91 | LR7 | TACTACCACCAAGATCT | 17 |
| 92 | Alphafor | CATTTTCCAACAGCACA | 17 |
| 93 | SV40-pAR | GAAATTTGTGATGCTATTGC | 20 |
| 94 | pE-C-R | CCTCACAAATGTGTATGG | 18 |
| 95 | U6 | CAGTGCAGGGGAAAGAATAGTAGAC | 25 |
| 96 | RAM14F | GTTGGATTATTTTAACCAATGCAGAT | 26 |
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